Construct: shRNA TRCN0000177016

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172725.1-1709s1c1
DNA Barcode:
GAACAGATCAACAAGTTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap5z1 (231855)
Gene Description:
adaptor-related protein complex 5, zeta 1 subunit
Transcript:
RefSeq NM_172725.1 (NON-CURRENT)
Match location:
Position 1709 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177016)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAGATCAACAAGTTCTTT
Hairpin Sequence:
5'-CCGG-GAACAGATCAACAAGTTCTTT-CTCGAG-AAAGAACTTGTTGATCTGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAGATCAACAAGTTCTTTCTCGAGAAAGAACTTGTTGATCTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAGATCAACAAGTTCTTTCTCGAGAAAGAACTTGTTGATCTGTTC-3'

Other clones with same target sequence:

(none)