Construct: shRNA TRCN0000177043
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_029231.2-285s1c1
- DNA Barcode:
- GCATTGGTGAATCTCAGTAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Pelp1 (75273)
- Gene Description:
- proline, glutamic acid and leucine rich protein 1
- Transcript:
- RefSeq NM_029231.2 (NON-CURRENT)
- Match location:
- Position 285 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000177043)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCATTGGTGAATCTCAGTAAT
- Hairpin Sequence:
-
5'-CCGG-GCATTGGTGAATCTCAGTAAT-CTCGAG-ATTACTGAGATTCACCAATGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCATTGGTGAATCTCAGTAATCTCGAGATTACTGAGATTCACCAATGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCATTGGTGAATCTCAGTAATCTCGAGATTACTGAGATTCACCAATGC-3'
Other clones with same target sequence:
-
TRCN0000292849
