Construct: shRNA TRCN0000177073

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172610.1-876s1c1
DNA Barcode:
GAAGGAGTAGACATCCTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mpped1 (223726)
Gene Description:
metallophosphoesterase domain containing 1
Transcript:
RefSeq NM_172610.1 (NON-CURRENT)
Match location:
Position 876 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177073)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGAGTAGACATCCTTATA
Hairpin Sequence:
5'-CCGG-GAAGGAGTAGACATCCTTATA-CTCGAG-TATAAGGATGTCTACTCCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGAGTAGACATCCTTATACTCGAGTATAAGGATGTCTACTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGAGTAGACATCCTTATACTCGAGTATAAGGATGTCTACTCCTTC-3'

Other clones with same target sequence:

(none)