Construct: shRNA TRCN0000177098

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172513.2-2553s1c1
DNA Barcode:
CCCAGGAATGATTGATATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam126b (213056)
Gene Description:
family with sequence similarity 126, member B
Transcript:
RefSeq NM_172513.2 (NON-CURRENT)
Match location:
Position 2553 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177098)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGAATGATTGATATTAT
Hairpin Sequence:
5'-CCGG-CCCAGGAATGATTGATATTAT-CTCGAG-ATAATATCAATCATTCCTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGAATGATTGATATTATCTCGAGATAATATCAATCATTCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGAATGATTGATATTATCTCGAGATAATATCAATCATTCCTGGG-3'

Other clones with same target sequence:

(none)