Construct: shRNA TRCN0000177139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175107.3-282s1c1
DNA Barcode:
GCTGTGATGTTTGTGGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2310022A10Rik (66367)
Gene Description:
RIKEN cDNA 2310022A10 gene
Transcript:
RefSeq NM_175107.3 (NON-CURRENT)
Match location:
Position 282 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGATGTTTGTGGATAAT
Hairpin Sequence:
5'-CCGG-GCTGTGATGTTTGTGGATAAT-CTCGAG-ATTATCCACAAACATCACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGATGTTTGTGGATAATCTCGAGATTATCCACAAACATCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGATGTTTGTGGATAATCTCGAGATTATCCACAAACATCACAGC-3'

Other clones with same target sequence:

(none)