Construct: shRNA TRCN0000177160

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175141.2-1198s1c1
DNA Barcode:
GCTTATTTAATCTTCCAGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1810030O07Rik (69155)
Gene Description:
RIKEN cDNA 1810030O07 gene
Transcript:
RefSeq NM_175141.2 (NON-CURRENT)
Match location:
Position 1198 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177160)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTATTTAATCTTCCAGGTT
Hairpin Sequence:
5'-CCGG-GCTTATTTAATCTTCCAGGTT-CTCGAG-AACCTGGAAGATTAAATAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTATTTAATCTTCCAGGTTCTCGAGAACCTGGAAGATTAAATAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTATTTAATCTTCCAGGTTCTCGAGAACCTGGAAGATTAAATAAGC-3'

Other clones with same target sequence:

(none)