Construct: shRNA TRCN0000177247

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029816.1-613s1c1
DNA Barcode:
GATTCTCATGCAGAACATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2610028H24Rik (76964)
Gene Description:
RIKEN cDNA 2610028H24 gene
Transcript:
RefSeq NM_029816.1 (NON-CURRENT)
Match location:
Position 613 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177247)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCTCATGCAGAACATGAT
Hairpin Sequence:
5'-CCGG-GATTCTCATGCAGAACATGAT-CTCGAG-ATCATGTTCTGCATGAGAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCTCATGCAGAACATGATCTCGAGATCATGTTCTGCATGAGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCTCATGCAGAACATGATCTCGAGATCATGTTCTGCATGAGAATC-3'

Other clones with same target sequence:

(none)