Construct: shRNA TRCN0000177249
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_030225.3-1608s1c1
- DNA Barcode:
- GCCTTAATGGATGCTCATTAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Dlst (78920)
- Gene Description:
- dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)
- Transcript:
- RefSeq NM_030225.3 (NON-CURRENT)
- Match location:
- Position 1608 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000177249)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTTAATGGATGCTCATTAA
- Hairpin Sequence:
-
5'-CCGG-GCCTTAATGGATGCTCATTAA-CTCGAG-TTAATGAGCATCCATTAAGGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTTAATGGATGCTCATTAACTCGAGTTAATGAGCATCCATTAAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTTAATGGATGCTCATTAACTCGAGTTAATGAGCATCCATTAAGGC-3'
Other clones with same target sequence:
(none)
