Construct: shRNA TRCN0000177253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153160.2-822s1c1
DNA Barcode:
GAAGAAGAAGAAGCACAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zcchc17 (619605)
Gene Description:
zinc finger, CCHC domain containing 17
Transcript:
RefSeq NM_153160.2 (NON-CURRENT)
Match location:
Position 822 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAAGAAGAAGCACAAGAA
Hairpin Sequence:
5'-CCGG-GAAGAAGAAGAAGCACAAGAA-CTCGAG-TTCTTGTGCTTCTTCTTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAAGAAGAAGCACAAGAACTCGAGTTCTTGTGCTTCTTCTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAAGAAGAAGCACAAGAACTCGAGTTCTTGTGCTTCTTCTTCTTC-3'

Other clones with same target sequence:

(none)