Construct: shRNA TRCN0000177283

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026251.1-597s1c1
DNA Barcode:
GATGAATGTGTGTGTTCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Patl2 (67578)
Gene Description:
protein associated with topoisomerase II homolog 2 (yeast)
Transcript:
RefSeq NM_026251.1 (NON-CURRENT)
Match location:
Position 597 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177283)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAATGTGTGTGTTCAGAT
Hairpin Sequence:
5'-CCGG-GATGAATGTGTGTGTTCAGAT-CTCGAG-ATCTGAACACACACATTCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAATGTGTGTGTTCAGATCTCGAGATCTGAACACACACATTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAATGTGTGTGTTCAGATCTCGAGATCTGAACACACACATTCATC-3'

Other clones with same target sequence:

(none)