Construct: shRNA TRCN0000177298

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172405.1-140s1c1
DNA Barcode:
GCCAAGAATAGCATTACTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abraxas1 (70681)
Gene Description:
BRCA1 A complex subunit
Transcript:
RefSeq NM_172405.1 (NON-CURRENT)
Match location:
Position 140 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177298)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAATAGCATTACTGAT
Hairpin Sequence:
5'-CCGG-GCCAAGAATAGCATTACTGAT-CTCGAG-ATCAGTAATGCTATTCTTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAATAGCATTACTGATCTCGAGATCAGTAATGCTATTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAATAGCATTACTGATCTCGAGATCAGTAATGCTATTCTTGGC-3'

Other clones with same target sequence:

(none)