Construct: shRNA TRCN0000177315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026201.1-552s1c1
DNA Barcode:
GCTACATGATACATTTGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccar1 (67500)
Gene Description:
cell division cycle and apoptosis regulator 1
Transcript:
RefSeq NM_026201.1 (NON-CURRENT)
Match location:
Position 552 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACATGATACATTTGGATT
Hairpin Sequence:
5'-CCGG-GCTACATGATACATTTGGATT-CTCGAG-AATCCAAATGTATCATGTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACATGATACATTTGGATTCTCGAGAATCCAAATGTATCATGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACATGATACATTTGGATTCTCGAGAATCCAAATGTATCATGTAGC-3'

Other clones with same target sequence:

(none)