Construct: shRNA TRCN0000177317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027464.1-431s1c1
DNA Barcode:
GATTTCCAACCTTACTTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam213a (70564)
Gene Description:
family with sequence similarity 213, member A
Transcript:
RefSeq NM_027464.1 (NON-CURRENT)
Match location:
Position 431 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTTCCAACCTTACTTCAAA
Hairpin Sequence:
5'-CCGG-GATTTCCAACCTTACTTCAAA-CTCGAG-TTTGAAGTAAGGTTGGAAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTTCCAACCTTACTTCAAACTCGAGTTTGAAGTAAGGTTGGAAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTTCCAACCTTACTTCAAACTCGAGTTTGAAGTAAGGTTGGAAATC-3'

Other clones with same target sequence:

  1. TRCN0000298073