Construct: shRNA TRCN0000177355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009529.1-298s1c1
DNA Barcode:
GCAGATGAAGATATAAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm4836 (22526)
Gene Description:
predicted gene 4836
Transcript:
RefSeq NM_009529.1 (NON-CURRENT)
Match location:
Position 298 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGAAGATATAAGAGAT
Hairpin Sequence:
5'-CCGG-GCAGATGAAGATATAAGAGAT-CTCGAG-ATCTCTTATATCTTCATCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGAAGATATAAGAGATCTCGAGATCTCTTATATCTTCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGAAGATATAAGAGATCTCGAGATCTCTTATATCTTCATCTGC-3'

Other clones with same target sequence:

(none)