Construct: shRNA TRCN0000177399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025816.1-2716s1c1
DNA Barcode:
GCTGTGACTACATACAATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tax1bp1 (52440)
Gene Description:
Tax1 (human T cell leukemia virus type I) binding protein 1
Transcript:
RefSeq NM_025816.1 (NON-CURRENT)
Match location:
Position 2716 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGACTACATACAATGAA
Hairpin Sequence:
5'-CCGG-GCTGTGACTACATACAATGAA-CTCGAG-TTCATTGTATGTAGTCACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGACTACATACAATGAACTCGAGTTCATTGTATGTAGTCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGACTACATACAATGAACTCGAGTTCATTGTATGTAGTCACAGC-3'

Other clones with same target sequence:

(none)