Construct: shRNA TRCN0000177658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133898.1-487s1c1
DNA Barcode:
CGCTGGAAATTCAACGTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
N4bp2l1 (100637)
Gene Description:
NEDD4 binding protein 2-like 1
Transcript:
RefSeq NM_133898.1 (NON-CURRENT)
Match location:
Position 487 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGGAAATTCAACGTTCAA
Hairpin Sequence:
5'-CCGG-CGCTGGAAATTCAACGTTCAA-CTCGAG-TTGAACGTTGAATTTCCAGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGGAAATTCAACGTTCAACTCGAGTTGAACGTTGAATTTCCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGGAAATTCAACGTTCAACTCGAGTTGAACGTTGAATTTCCAGCG-3'

Other clones with same target sequence:

(none)