Construct: shRNA TRCN0000177698

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027401.1-586s1c1
DNA Barcode:
CAGACGGATACATTTGCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam135b (70363)
Gene Description:
family with sequence similarity 135, member B
Transcript:
RefSeq NM_027401.1 (NON-CURRENT)
Match location:
Position 586 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177698)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGACGGATACATTTGCAGAT
Hairpin Sequence:
5'-CCGG-CAGACGGATACATTTGCAGAT-CTCGAG-ATCTGCAAATGTATCCGTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGACGGATACATTTGCAGATCTCGAGATCTGCAAATGTATCCGTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGACGGATACATTTGCAGATCTCGAGATCTGCAAATGTATCCGTCTG-3'

Other clones with same target sequence:

(none)