Construct: shRNA TRCN0000177724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172503.2-2507s1c1
DNA Barcode:
CATGAAGAACTGGTACTCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zswim4 (212168)
Gene Description:
zinc finger SWIM-type containing 4
Transcript:
RefSeq NM_172503.2 (NON-CURRENT)
Match location:
Position 2507 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAAGAACTGGTACTCCTT
Hairpin Sequence:
5'-CCGG-CATGAAGAACTGGTACTCCTT-CTCGAG-AAGGAGTACCAGTTCTTCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAAGAACTGGTACTCCTTCTCGAGAAGGAGTACCAGTTCTTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAAGAACTGGTACTCCTTCTCGAGAAGGAGTACCAGTTCTTCATG-3'

Other clones with same target sequence:

(none)