Construct: shRNA TRCN0000177766

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026150.1-390s1c1
DNA Barcode:
CAAGAGGAAGATGAGCTACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4921536K21Rik (67430)
Gene Description:
RIKEN cDNA 4921536K21 gene
Transcript:
RefSeq NM_026150.1 (NON-CURRENT)
Match location:
Position 390 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177766)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAGGAAGATGAGCTACTT
Hairpin Sequence:
5'-CCGG-CAAGAGGAAGATGAGCTACTT-CTCGAG-AAGTAGCTCATCTTCCTCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAGGAAGATGAGCTACTTCTCGAGAAGTAGCTCATCTTCCTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAGGAAGATGAGCTACTTCTCGAGAAGTAGCTCATCTTCCTCTTG-3'

Other clones with same target sequence:

(none)