Construct: shRNA TRCN0000177783

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172743.1-1893s1c1
DNA Barcode:
CATTGATGTCAAGCTGAGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plekha7 (233765)
Gene Description:
pleckstrin homology domain containing, family A member 7
Transcript:
RefSeq NM_172743.1 (NON-CURRENT)
Match location:
Position 1893 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177783)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTGATGTCAAGCTGAGCAT
Hairpin Sequence:
5'-CCGG-CATTGATGTCAAGCTGAGCAT-CTCGAG-ATGCTCAGCTTGACATCAATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTGATGTCAAGCTGAGCATCTCGAGATGCTCAGCTTGACATCAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTGATGTCAAGCTGAGCATCTCGAGATGCTCAGCTTGACATCAATG-3'

Other clones with same target sequence:

  1. TRCN0000277178