Construct: shRNA TRCN0000177794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011225.1-397s1c1
DNA Barcode:
CGATAGAAATGAAGGCTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab18 (19330)
Gene Description:
RAB18, member RAS oncogene family
Transcript:
RefSeq NM_011225.1 (NON-CURRENT)
Match location:
Position 397 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATAGAAATGAAGGCTTGAA
Hairpin Sequence:
5'-CCGG-CGATAGAAATGAAGGCTTGAA-CTCGAG-TTCAAGCCTTCATTTCTATCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATAGAAATGAAGGCTTGAACTCGAGTTCAAGCCTTCATTTCTATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATAGAAATGAAGGCTTGAACTCGAGTTCAAGCCTTCATTTCTATCG-3'

Other clones with same target sequence:

(none)