Construct: shRNA TRCN0000177802

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029784.1-1114s1c1
DNA Barcode:
CAATGAAAGAGACGTAGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam81a (76886)
Gene Description:
family with sequence similarity 81, member A
Transcript:
RefSeq NM_029784.1 (NON-CURRENT)
Match location:
Position 1114 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177802)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATGAAAGAGACGTAGAGAA
Hairpin Sequence:
5'-CCGG-CAATGAAAGAGACGTAGAGAA-CTCGAG-TTCTCTACGTCTCTTTCATTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATGAAAGAGACGTAGAGAACTCGAGTTCTCTACGTCTCTTTCATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATGAAAGAGACGTAGAGAACTCGAGTTCTCTACGTCTCTTTCATTG-3'

Other clones with same target sequence:

(none)