Construct: shRNA TRCN0000177815

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001029893.1-443s1c1
DNA Barcode:
CGGCAGAGAGACATTATACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psg26 (574429)
Gene Description:
pregnancy-specific glycoprotein 26
Transcript:
RefSeq NM_001029893.1 (CURRENT)
Match location:
Position 443 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177815)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAGAGAGACATTATACAT
Hairpin Sequence:
5'-CCGG-CGGCAGAGAGACATTATACAT-CTCGAG-ATGTATAATGTCTCTCTGCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAGAGAGACATTATACATCTCGAGATGTATAATGTCTCTCTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAGAGAGACATTATACATCTCGAGATGTATAATGTCTCTCTGCCG-3'

Other clones with same target sequence:

(none)