Construct: shRNA TRCN0000177899

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145604.1-1944s1c1
DNA Barcode:
CCTAAGTGGATGAAATCGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D230025D16Rik (234678)
Gene Description:
RIKEN cDNA D230025D16 gene
Transcript:
RefSeq NM_145604.1 (NON-CURRENT)
Match location:
Position 1944 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177899)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAGTGGATGAAATCGTTT
Hairpin Sequence:
5'-CCGG-CCTAAGTGGATGAAATCGTTT-CTCGAG-AAACGATTTCATCCACTTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAGTGGATGAAATCGTTTCTCGAGAAACGATTTCATCCACTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAGTGGATGAAATCGTTTCTCGAGAAACGATTTCATCCACTTAGG-3'

Other clones with same target sequence:

(none)