Construct: shRNA TRCN0000177905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172274.1-1291s1c1
DNA Barcode:
CCAAGATTCCTGGAAGATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cc2d2a (231214)
Gene Description:
coiled-coil and C2 domain containing 2A
Transcript:
RefSeq NM_172274.1 (NON-CURRENT)
Match location:
Position 1291 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGATTCCTGGAAGATGAA
Hairpin Sequence:
5'-CCGG-CCAAGATTCCTGGAAGATGAA-CTCGAG-TTCATCTTCCAGGAATCTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGATTCCTGGAAGATGAACTCGAGTTCATCTTCCAGGAATCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGATTCCTGGAAGATGAACTCGAGTTCATCTTCCAGGAATCTTGG-3'

Other clones with same target sequence:

(none)