Construct: shRNA TRCN0000177915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201368.1-882s1c1
DNA Barcode:
CATCCTCTATTTCTCCTGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xkr8 (381560)
Gene Description:
X-linked Kx blood group related 8
Transcript:
RefSeq NM_201368.1 (CURRENT)
Match location:
Position 882 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCTCTATTTCTCCTGGTT
Hairpin Sequence:
5'-CCGG-CATCCTCTATTTCTCCTGGTT-CTCGAG-AACCAGGAGAAATAGAGGATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCTCTATTTCTCCTGGTTCTCGAGAACCAGGAGAAATAGAGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCTCTATTTCTCCTGGTTCTCGAGAACCAGGAGAAATAGAGGATG-3'

Other clones with same target sequence:

(none)