Construct: shRNA TRCN0000177942

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026034.1-1013s1c1
DNA Barcode:
GCTTTAGCAATAAAGCCGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Armc10 (67211)
Gene Description:
armadillo repeat containing 10
Transcript:
RefSeq NM_026034.1 (NON-CURRENT)
Match location:
Position 1013 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177942)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTAGCAATAAAGCCGAAA
Hairpin Sequence:
5'-CCGG-GCTTTAGCAATAAAGCCGAAA-CTCGAG-TTTCGGCTTTATTGCTAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTAGCAATAAAGCCGAAACTCGAGTTTCGGCTTTATTGCTAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTAGCAATAAAGCCGAAACTCGAGTTTCGGCTTTATTGCTAAAGC-3'

Other clones with same target sequence:

(none)