Construct: shRNA TRCN0000177958

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177164.2-847s1c1
DNA Barcode:
GAAGACTATCCTGCTGATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vwc2l (320460)
Gene Description:
von Willebrand factor C domain-containing protein 2-like
Transcript:
RefSeq NM_177164.2 (NON-CURRENT)
Match location:
Position 847 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177958)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGACTATCCTGCTGATGAA
Hairpin Sequence:
5'-CCGG-GAAGACTATCCTGCTGATGAA-CTCGAG-TTCATCAGCAGGATAGTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGACTATCCTGCTGATGAACTCGAGTTCATCAGCAGGATAGTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGACTATCCTGCTGATGAACTCGAGTTCATCAGCAGGATAGTCTTC-3'

Other clones with same target sequence:

(none)