Construct: shRNA TRCN0000178070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025712.2-1121s1c1
DNA Barcode:
GCCAGCAGTGGTTTGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snx31 (66696)
Gene Description:
sorting nexin 31
Transcript:
RefSeq NM_025712.2 (NON-CURRENT)
Match location:
Position 1121 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCAGTGGTTTGTTATTT
Hairpin Sequence:
5'-CCGG-GCCAGCAGTGGTTTGTTATTT-CTCGAG-AAATAACAAACCACTGCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCAGTGGTTTGTTATTTCTCGAGAAATAACAAACCACTGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCAGTGGTTTGTTATTTCTCGAGAAATAACAAACCACTGCTGGC-3'

Other clones with same target sequence:

(none)