Construct: shRNA TRCN0000178091

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175258.3-1145s1c1
DNA Barcode:
GCTTGACTCATGGTATGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rapgef6 (192786)
Gene Description:
Rap guanine nucleotide exchange factor (GEF) 6
Transcript:
RefSeq NM_175258.3 (NON-CURRENT)
Match location:
Position 1145 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178091)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGACTCATGGTATGTCAT
Hairpin Sequence:
5'-CCGG-GCTTGACTCATGGTATGTCAT-CTCGAG-ATGACATACCATGAGTCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGACTCATGGTATGTCATCTCGAGATGACATACCATGAGTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGACTCATGGTATGTCATCTCGAGATGACATACCATGAGTCAAGC-3'

Other clones with same target sequence:

  1. TRCN0000346973