Construct: shRNA TRCN0000178177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172286.2-1278s1c1
DNA Barcode:
GAAGAGCAATACAAGCTGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
6430548M08Rik (234797)
Gene Description:
RIKEN cDNA 6430548M08 gene
Transcript:
RefSeq NM_172286.2 (NON-CURRENT)
Match location:
Position 1278 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAGCAATACAAGCTGCTA
Hairpin Sequence:
5'-CCGG-GAAGAGCAATACAAGCTGCTA-CTCGAG-TAGCAGCTTGTATTGCTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAGCAATACAAGCTGCTACTCGAGTAGCAGCTTGTATTGCTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAGCAATACAAGCTGCTACTCGAGTAGCAGCTTGTATTGCTCTTC-3'

Other clones with same target sequence:

(none)