Construct: shRNA TRCN0000178223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134034.1-685s1c1
DNA Barcode:
GCCAGAAACTAGTCATCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp4r3b (104570)
Gene Description:
protein phosphatase 4 regulatory subunit 3B
Transcript:
RefSeq NM_134034.1 (NON-CURRENT)
Match location:
Position 685 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAAACTAGTCATCTAAT
Hairpin Sequence:
5'-CCGG-GCCAGAAACTAGTCATCTAAT-CTCGAG-ATTAGATGACTAGTTTCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAAACTAGTCATCTAATCTCGAGATTAGATGACTAGTTTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAAACTAGTCATCTAATCTCGAGATTAGATGACTAGTTTCTGGC-3'

Other clones with same target sequence:

(none)