Construct: shRNA TRCN0000178311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007414.2-832s1c1
DNA Barcode:
GAATCTTCAACACTGGTCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adprh (11544)
Gene Description:
ADP-ribosylarginine hydrolase
Transcript:
RefSeq NM_007414.2 (NON-CURRENT)
Match location:
Position 832 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATCTTCAACACTGGTCCTA
Hairpin Sequence:
5'-CCGG-GAATCTTCAACACTGGTCCTA-CTCGAG-TAGGACCAGTGTTGAAGATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATCTTCAACACTGGTCCTACTCGAGTAGGACCAGTGTTGAAGATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATCTTCAACACTGGTCCTACTCGAGTAGGACCAGTGTTGAAGATTC-3'

Other clones with same target sequence:

(none)