Construct: shRNA TRCN0000178345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178897.2-349s1c1
DNA Barcode:
GATCCAGATGACAGTCTGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tyw1 (100929)
Gene Description:
tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)
Transcript:
RefSeq NM_178897.2 (NON-CURRENT)
Match location:
Position 349 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCAGATGACAGTCTGATA
Hairpin Sequence:
5'-CCGG-GATCCAGATGACAGTCTGATA-CTCGAG-TATCAGACTGTCATCTGGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCAGATGACAGTCTGATACTCGAGTATCAGACTGTCATCTGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCAGATGACAGTCTGATACTCGAGTATCAGACTGTCATCTGGATC-3'

Other clones with same target sequence:

(none)