Construct: shRNA TRCN0000178394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031408.1-85s1c1
DNA Barcode:
GAACTACAGGACAAGGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gigyf1 (57330)
Gene Description:
GRB10 interacting GYF protein 1
Transcript:
RefSeq NM_031408.1 (NON-CURRENT)
Match location:
Position 85 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTACAGGACAAGGAGTTT
Hairpin Sequence:
5'-CCGG-GAACTACAGGACAAGGAGTTT-CTCGAG-AAACTCCTTGTCCTGTAGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTACAGGACAAGGAGTTTCTCGAGAAACTCCTTGTCCTGTAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTACAGGACAAGGAGTTTCTCGAGAAACTCCTTGTCCTGTAGTTC-3'

Other clones with same target sequence:

  1. TRCN0000340965