Construct: shRNA TRCN0000178406

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198296.1-272s1c1
DNA Barcode:
GCTGAAGTTCCTTATGTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9130011E15Rik (71617)
Gene Description:
RIKEN cDNA 9130011E15 gene
Transcript:
RefSeq NM_198296.1 (NON-CURRENT)
Match location:
Position 272 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178406)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAGTTCCTTATGTCAAA
Hairpin Sequence:
5'-CCGG-GCTGAAGTTCCTTATGTCAAA-CTCGAG-TTTGACATAAGGAACTTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAGTTCCTTATGTCAAACTCGAGTTTGACATAAGGAACTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAGTTCCTTATGTCAAACTCGAGTTTGACATAAGGAACTTCAGC-3'

Other clones with same target sequence:

(none)