Construct: shRNA TRCN0000178480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025581.2-596s1c1
DNA Barcode:
GCGTACATGAAATCCCGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ska1 (66468)
Gene Description:
spindle and kinetochore associated complex subunit 1
Transcript:
RefSeq NM_025581.2 (NON-CURRENT)
Match location:
Position 596 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTACATGAAATCCCGTTTA
Hairpin Sequence:
5'-CCGG-GCGTACATGAAATCCCGTTTA-CTCGAG-TAAACGGGATTTCATGTACGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTACATGAAATCCCGTTTACTCGAGTAAACGGGATTTCATGTACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTACATGAAATCCCGTTTACTCGAGTAAACGGGATTTCATGTACGC-3'

Other clones with same target sequence:

  1. TRCN0000340464