Construct: shRNA TRCN0000178490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177801.2-727s1c1
DNA Barcode:
GAAGTCCCAGAATACCTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spata32 (328019)
Gene Description:
spermatogenesis associated 32
Transcript:
RefSeq NM_177801.2 (NON-CURRENT)
Match location:
Position 727 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTCCCAGAATACCTCTTT
Hairpin Sequence:
5'-CCGG-GAAGTCCCAGAATACCTCTTT-CTCGAG-AAAGAGGTATTCTGGGACTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTCCCAGAATACCTCTTTCTCGAGAAAGAGGTATTCTGGGACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTCCCAGAATACCTCTTTCTCGAGAAAGAGGTATTCTGGGACTTC-3'

Other clones with same target sequence:

(none)