Construct: shRNA TRCN0000178526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026164.1-253s1c1
DNA Barcode:
GCAAGAAGTCTTTGTGGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pnpla8 (67452)
Gene Description:
patatin-like phospholipase domain containing 8
Transcript:
RefSeq NM_026164.1 (NON-CURRENT)
Match location:
Position 253 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGAAGTCTTTGTGGGAAA
Hairpin Sequence:
5'-CCGG-GCAAGAAGTCTTTGTGGGAAA-CTCGAG-TTTCCCACAAAGACTTCTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGAAGTCTTTGTGGGAAACTCGAGTTTCCCACAAAGACTTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGAAGTCTTTGTGGGAAACTCGAGTTTCCCACAAAGACTTCTTGC-3'

Other clones with same target sequence:

  1. TRCN0000345749