Construct: shRNA TRCN0000178543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175107.3-1954s1c1
DNA Barcode:
GCGAACCTGTAAAGACTGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2310022A10Rik (66367)
Gene Description:
RIKEN cDNA 2310022A10 gene
Transcript:
RefSeq NM_175107.3 (NON-CURRENT)
Match location:
Position 1954 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAACCTGTAAAGACTGTTA
Hairpin Sequence:
5'-CCGG-GCGAACCTGTAAAGACTGTTA-CTCGAG-TAACAGTCTTTACAGGTTCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAACCTGTAAAGACTGTTACTCGAGTAACAGTCTTTACAGGTTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAACCTGTAAAGACTGTTACTCGAGTAACAGTCTTTACAGGTTCGC-3'

Other clones with same target sequence:

  1. TRCN0000277373