Construct: shRNA TRCN0000178580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026188.1-395s1c1
DNA Barcode:
CAAAGACTTGGTGGAGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1700028P14Rik (67483)
Gene Description:
RIKEN cDNA 1700028P14 gene
Transcript:
RefSeq NM_026188.1 (NON-CURRENT)
Match location:
Position 395 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGACTTGGTGGAGAAGAA
Hairpin Sequence:
5'-CCGG-CAAAGACTTGGTGGAGAAGAA-CTCGAG-TTCTTCTCCACCAAGTCTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGACTTGGTGGAGAAGAACTCGAGTTCTTCTCCACCAAGTCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGACTTGGTGGAGAAGAACTCGAGTTCTTCTCCACCAAGTCTTTG-3'

Other clones with same target sequence:

(none)