Construct: shRNA TRCN0000178641

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025386.1-649s1c1
DNA Barcode:
CCAAAGCTCACTCTCTCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxo36 (66153)
Gene Description:
F-box protein 36
Transcript:
RefSeq NM_025386.1 (NON-CURRENT)
Match location:
Position 649 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178641)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGCTCACTCTCTCTTAA
Hairpin Sequence:
5'-CCGG-CCAAAGCTCACTCTCTCTTAA-CTCGAG-TTAAGAGAGAGTGAGCTTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGCTCACTCTCTCTTAACTCGAGTTAAGAGAGAGTGAGCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGCTCACTCTCTCTTAACTCGAGTTAAGAGAGAGTGAGCTTTGG-3'

Other clones with same target sequence:

  1. TRCN0000292963