Construct: shRNA TRCN0000178649

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173379.1-1207s1c1
DNA Barcode:
CGTCATAAACTTGAAGCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
P3h2 (210530)
Gene Description:
prolyl 3-hydroxylase 2
Transcript:
RefSeq NM_173379.1 (NON-CURRENT)
Match location:
Position 1207 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178649)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCATAAACTTGAAGCTGAA
Hairpin Sequence:
5'-CCGG-CGTCATAAACTTGAAGCTGAA-CTCGAG-TTCAGCTTCAAGTTTATGACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCATAAACTTGAAGCTGAACTCGAGTTCAGCTTCAAGTTTATGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCATAAACTTGAAGCTGAACTCGAGTTCAGCTTCAAGTTTATGACG-3'

Other clones with same target sequence:

(none)