Construct: shRNA TRCN0000178657

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001488.2-4595s1c1
DNA Barcode:
CCTGTTTCTTTCTACCAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp8b1 (54670)
Gene Description:
ATPase, class I, type 8B, member 1
Transcript:
RefSeq NM_001001488.2 (NON-CURRENT)
Match location:
Position 4595 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178657)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTTCTTTCTACCAACTT
Hairpin Sequence:
5'-CCGG-CCTGTTTCTTTCTACCAACTT-CTCGAG-AAGTTGGTAGAAAGAAACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTTCTTTCTACCAACTTCTCGAGAAGTTGGTAGAAAGAAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTTCTTTCTACCAACTTCTCGAGAAGTTGGTAGAAAGAAACAGG-3'

Other clones with same target sequence:

(none)