Construct: shRNA TRCN0000178677

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172414.1-250s1c1
DNA Barcode:
CTTACGAACAAAGTGACTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zc2hc1c (72350)
Gene Description:
zinc finger, C2HC-type containing 1C
Transcript:
RefSeq NM_172414.1 (NON-CURRENT)
Match location:
Position 250 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178677)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTACGAACAAAGTGACTCTT
Hairpin Sequence:
5'-CCGG-CTTACGAACAAAGTGACTCTT-CTCGAG-AAGAGTCACTTTGTTCGTAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTACGAACAAAGTGACTCTTCTCGAGAAGAGTCACTTTGTTCGTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTACGAACAAAGTGACTCTTCTCGAGAAGAGTCACTTTGTTCGTAAG-3'

Other clones with same target sequence:

(none)