Construct: shRNA TRCN0000178695

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025818.1-435s1c1
DNA Barcode:
CCAGGATATCTTCTCCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ncbp3 (66874)
Gene Description:
nuclear cap binding subunit 3
Transcript:
RefSeq NM_025818.1 (NON-CURRENT)
Match location:
Position 435 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178695)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGATATCTTCTCCTATTT
Hairpin Sequence:
5'-CCGG-CCAGGATATCTTCTCCTATTT-CTCGAG-AAATAGGAGAAGATATCCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGATATCTTCTCCTATTTCTCGAGAAATAGGAGAAGATATCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGATATCTTCTCCTATTTCTCGAGAAATAGGAGAAGATATCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000336400