Construct: shRNA TRCN0000178699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026522.2-675s1c1
DNA Barcode:
CAGAAACATGTAGGCCTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chid1 (68038)
Gene Description:
chitinase domain containing 1
Transcript:
RefSeq NM_026522.2 (NON-CURRENT)
Match location:
Position 675 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAAACATGTAGGCCTCATT
Hairpin Sequence:
5'-CCGG-CAGAAACATGTAGGCCTCATT-CTCGAG-AATGAGGCCTACATGTTTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAAACATGTAGGCCTCATTCTCGAGAATGAGGCCTACATGTTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAAACATGTAGGCCTCATTCTCGAGAATGAGGCCTACATGTTTCTG-3'

Other clones with same target sequence:

  1. TRCN0000276899