Construct: shRNA TRCN0000178729

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006371.2-877s1c1
DNA Barcode:
CGGTTGAGAAATTTGTGGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRTAP (10491)
Gene Description:
cartilage associated protein
Transcript:
RefSeq NM_006371.2 (NON-CURRENT)
Match location:
Position 877 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178729)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTGAGAAATTTGTGGCTA
Hairpin Sequence:
5'-CCGG-CGGTTGAGAAATTTGTGGCTA-CTCGAG-TAGCCACAAATTTCTCAACCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTGAGAAATTTGTGGCTACTCGAGTAGCCACAAATTTCTCAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTGAGAAATTTGTGGCTACTCGAGTAGCCACAAATTTCTCAACCG-3'

Other clones with same target sequence:

(none)