Construct: shRNA TRCN0000178745

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145940.1-743s1c1
DNA Barcode:
CTATGAGTTTCGTCGAGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wipi1 (52639)
Gene Description:
WD repeat domain, phosphoinositide interacting 1
Transcript:
RefSeq NM_145940.1 (NON-CURRENT)
Match location:
Position 743 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178745)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGAGTTTCGTCGAGGAAT
Hairpin Sequence:
5'-CCGG-CTATGAGTTTCGTCGAGGAAT-CTCGAG-ATTCCTCGACGAAACTCATAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGAGTTTCGTCGAGGAATCTCGAGATTCCTCGACGAAACTCATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGAGTTTCGTCGAGGAATCTCGAGATTCCTCGACGAAACTCATAG-3'

Other clones with same target sequence:

(none)