Construct: shRNA TRCN0000178779

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017710.1-1655s1c1
DNA Barcode:
CCATGAATCTCCATCAGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GON4L (54856)
Gene Description:
gon-4 like
Transcript:
RefSeq NM_017710.1 (NON-CURRENT)
Match location:
Position 1655 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178779)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGAATCTCCATCAGGAAT
Hairpin Sequence:
5'-CCGG-CCATGAATCTCCATCAGGAAT-CTCGAG-ATTCCTGATGGAGATTCATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGAATCTCCATCAGGAATCTCGAGATTCCTGATGGAGATTCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGAATCTCCATCAGGAATCTCGAGATTCCTGATGGAGATTCATGG-3'

Other clones with same target sequence:

(none)